Mouse Anti-CA9 Recombinant Antibody (clone M75)

Customer Review

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Jessica Lewis
12.Mar,24

Great for CA9 Research

The Anti-CA9 Recombinant Antibody (clone M75) has been a fantastic addition to our CA9 studies. Its specificity and sensitivity are outstanding, consistently providing clear results. This antibody has become essential for our research, improving the accuracy and reliability of our assays.

Brian Wright
11.Apr,23

Reliable for Immunoassays

We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for CA9 is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.

Sarah Harris
14.Sep,22

Consistent and Effective

This antibody has proven to be consistent and effective in our various assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for CA9 have been particularly beneficial.

Q&As

1. Can the anti-CA9 recombinant antibody clone M75 be used in flow cytometry?

   A: Yes, the anti-CA9 recombinant antibody clone M75 (PABZ-017) can be used in flow cytometry. It is designed to specifically bind to CA9, making it suitable for detecting CA9-expressing cells in flow cytometry.

2. What are the storage recommendations for the anti-CA9 recombinant antibody clone M75?

   A: The recommended storage condition for the anti-CA9 recombinant antibody clone M75 (PABZ-017) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

3. Is the anti-CA9 recombinant antibody clone M75 suitable for use in immunoprecipitation assays?

   A: Yes, the anti-CA9 recombinant antibody clone M75 (PABZ-017) is suitable for use in immunoprecipitation assays. It provides specific binding to CA9, enabling the successful precipitation of the target protein from complex mixtures.

4. Can the anti-CA9 recombinant antibody clone M75 be used in ELISA applications?

   A: Yes, the anti-CA9 recombinant antibody clone M75 (PABZ-017) can be used in ELISA applications. It has been validated for use in such assays and provides reliable detection of CA9.

5. What is the optimal dilution for using the anti-CA9 recombinant antibody clone M75 in Western blotting?

   A: The optimal dilution for using the anti-CA9 recombinant antibody clone M75 (PABZ-017) in Western blotting is typically 1:500 to 1:2000. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

1. Genovesi, Laura A., et al. "Patient-derived orthotopic xenograft models of medulloblastoma lack a functional blood-brain barrier." Neuro-oncology 23.5 (2021): 732-742. https://doi.org/10.1093/neuonc/noaa266
   This study evaluates the integrity and permeability of the blood-brain-tumor barrier (BBTB) in various preclinical models of medulloblastoma (MB), the most common malignant pediatric brain tumor. The researchers utilized high-resolution magnetic resonance imaging (MRI) and pharmacokinetic modeling to compare the BBTB in genetically engineered mouse models (GEMMs) and patient-derived orthotopic xenograft (PDOX) models. They discovered significant differences in BBTB integrity between models, with GEMM models showing intact BBTB, while PDOX models displayed disrupted BBTB. The study highlights the importance of considering BBTB integrity in preclinical assessments to improve the translation of novel therapeutics into clinical settings.
   Creative Biolabs provided essential reagents for this research, specifically the anti-carbonic anhydrase IX (CAIX) antibody (Cat# PABZ-017). This antibody was crucial for immunohistochemistry analysis, enabling the researchers to identify and quantify hypoxic regions within tumor tissues. By providing this high-quality reagent, Creative Biolabs facilitated the study's exploration of the relationship between hypoxia and BBTB integrity, thereby supporting the overall objective of enhancing therapeutic strategies for medulloblastoma.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PABZ-017, RRID: AB_3111706)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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